BIOSYNTHESIS OF SILVER NANOPARTICLES AND CATHARANTUS ROSES AND THEIR ANTIBACTERIAL EFFICACY /
Oyawale, Anuoluwapo Modupe
BIOSYNTHESIS OF SILVER NANOPARTICLES AND CATHARANTUS ROSES AND THEIR ANTIBACTERIAL EFFICACY / ANUOLUWAPO MODUPE OYAWALE; SUPERVISOR: ASST. PROF. DR. PWADUBASHIYI COSTON PWAVODI - x, 52 sheets: charts, photos; 31 cm. Includes CD
Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
Includes bibliography (sheets 47-52)
ABSTRACT
The methodology for creating silver nanoparticles (AgNPs) is developed in this paper
by reducing Catharanthus roseus plant leaf extract. This research creates synthetic
nanoparticles with highly effective antibacterial properties that are controlled by the
manufacturing method. Smaller size, appropriate shape, evenly distribution, and the
creation of functional groups are some of these characteristics by enhancing the
parameters used in the reduction procedure. C. roseus leaf extract and AgNO3 were
used to undertake the biosynthesis of AgNPs. Silver nitrate of 0.6 g was mixed
with distilled water of 500 ml, the ideal conditions and concentration for the creation
of nanoparticles were created. A magnetic stirrer was used to agitate the liquid for 5
minutes. After that, Catharanthus roseus extracts were added carefully until the colour
changes from white to brownish occurred, showing the presence of synthesized AgNps
with Catharanthus roseus. After then, the mixture was stirred for 24 hours. The
samples were characterized using a variety of methods, including UV-Vis
spectrophotometer analysis, Fourier transforms infrared, X-ray diffraction
spectroscopy and Scanning electron microscopy. The size of 88 nm, spherical
morphology, functional group, absorbance rate, and crystallinity were all validated by
the findings of the characterization process. In the current study, zone of inhibition
methods was done to assess the anti-bacterial property of the synthesized silver
nanoparticle (AgNPs) against two distinct bacteria at doses of AgNPs (10 g/ml) alone
as well as in combination with the antibiotic gentamicin. Lastly, antioxidant activity
(DPPH radical scavenging method) was also assessed. According to the findings,
bacterial growth is inhibited by AgNPs at high concentrations. Additionally, against
bacteria that are resistant to antibiotics, AgNPs had a substantially better antibacterial
effect when combined with gentamicin. A practical and environmentally friendly
approach for creating silver nanoparticles has been devised utilizing Cantharantus
roseus leaf extract. In the near future, the fields of water treatment, biomedicine, and
nanotechnology may all benefit from the use of synthesized AgNPs.
Keywords; Antimicrobial activity, Antioxidant, Biosynthesis, Catharanthus roseus,
Characterization, Silver nanoparticles.
Anti-infective agents--Dissertations, Academic
Antioxidants--Dissertations, Academic
Biosynthesis--Dissertations, Academic
Catharanthus roseus--Dissertations, Academic
Nanoparticles--Dissertations, Academic
BIOSYNTHESIS OF SILVER NANOPARTICLES AND CATHARANTUS ROSES AND THEIR ANTIBACTERIAL EFFICACY / ANUOLUWAPO MODUPE OYAWALE; SUPERVISOR: ASST. PROF. DR. PWADUBASHIYI COSTON PWAVODI - x, 52 sheets: charts, photos; 31 cm. Includes CD
Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
Includes bibliography (sheets 47-52)
ABSTRACT
The methodology for creating silver nanoparticles (AgNPs) is developed in this paper
by reducing Catharanthus roseus plant leaf extract. This research creates synthetic
nanoparticles with highly effective antibacterial properties that are controlled by the
manufacturing method. Smaller size, appropriate shape, evenly distribution, and the
creation of functional groups are some of these characteristics by enhancing the
parameters used in the reduction procedure. C. roseus leaf extract and AgNO3 were
used to undertake the biosynthesis of AgNPs. Silver nitrate of 0.6 g was mixed
with distilled water of 500 ml, the ideal conditions and concentration for the creation
of nanoparticles were created. A magnetic stirrer was used to agitate the liquid for 5
minutes. After that, Catharanthus roseus extracts were added carefully until the colour
changes from white to brownish occurred, showing the presence of synthesized AgNps
with Catharanthus roseus. After then, the mixture was stirred for 24 hours. The
samples were characterized using a variety of methods, including UV-Vis
spectrophotometer analysis, Fourier transforms infrared, X-ray diffraction
spectroscopy and Scanning electron microscopy. The size of 88 nm, spherical
morphology, functional group, absorbance rate, and crystallinity were all validated by
the findings of the characterization process. In the current study, zone of inhibition
methods was done to assess the anti-bacterial property of the synthesized silver
nanoparticle (AgNPs) against two distinct bacteria at doses of AgNPs (10 g/ml) alone
as well as in combination with the antibiotic gentamicin. Lastly, antioxidant activity
(DPPH radical scavenging method) was also assessed. According to the findings,
bacterial growth is inhibited by AgNPs at high concentrations. Additionally, against
bacteria that are resistant to antibiotics, AgNPs had a substantially better antibacterial
effect when combined with gentamicin. A practical and environmentally friendly
approach for creating silver nanoparticles has been devised utilizing Cantharantus
roseus leaf extract. In the near future, the fields of water treatment, biomedicine, and
nanotechnology may all benefit from the use of synthesized AgNPs.
Keywords; Antimicrobial activity, Antioxidant, Biosynthesis, Catharanthus roseus,
Characterization, Silver nanoparticles.
Anti-infective agents--Dissertations, Academic
Antioxidants--Dissertations, Academic
Biosynthesis--Dissertations, Academic
Catharanthus roseus--Dissertations, Academic
Nanoparticles--Dissertations, Academic