PHYTOCHEMICAL SCREENING AND SYNTHESIS OF SILVER NANOPARTICLES AND INVESTIGATION OF BIOLOGICAL ACTIVITIES OF Silybum marianum LEAF IMMACULATA AMARACHI UNEGBU; SUPERVISOR: ASST. PROF. DR. EMMANUEL MSHELIA HALILU

Yazar: Katkıda bulunan(lar):Dil: İngilizce 2023Tanım: xii, 84 sheets; 31 cm. 1 CD-ROMİçerik türü:
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Ortam türü:
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Taşıyıcı türü:
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Konu(lar): Tez notu: Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department Özet: ABSTRACT Silybum marianum is a therapeutic plant belonging to the family Asteraceae. It contains silymarin, as its principal component, and has been reported to demonstrate hepatoprotective activity. The study was aimed to investigate the phytochemical constituents, synthesis of nanoparticles, antioxidant activity and antimicrobial effects of the leaf extract. The physiochemical analysis revealed moisture content, total ash, acid insoluble, alcohol, water, and petroleum ether soluble extraction of 13.9 %, 1.35 %, 0.95 %, 5.8 %, 7.1 % and 0.8 % respectively. The chemical profile was evaluated by Gas Chromatography-Mass Spectrometry (GC-MS) assay and qualitative phytochemical. Extracts were synthesized from AgNO3 and characterized via FT-IR, U.V and XRD. The antioxidant activity was determined using DPPH, H2O2 and βcarotene assays. Similarly, all extracts expressed significant DPPH inhibition (IC50) of 5.1, 5.0, 4.7, 2.3, 4.3 and 1.1 (ug/mL), H2O2 of 1.3, 2.2, 4.7, 5.6, 4.0 and 1.0 (ug/mL) from the methanol/water extract, nanoparticles, n-Hexane, ETOAC, n-Butanol and Ascorbic acid (standard) respectively. The extract exerted scavenging activity on βcarotene with a significant IC50 inhibition of 1.5, 0.8, 5.2, 0.8, 2.7, 4.8 at 0 hour and 1.5, 0.8, 5.2, 0.8, 2.7 and 4.8 (ug/mL) after 2 hours water bath incubation at 50°C in the order of the extracts above. Additionally, nanoparticles and plant extracts significantly (p<0.0001) inhibited the growth of bacteria with mean zone of inhibition ranging 7-13 mm and 4-11 mm. This study demonstrated that extracts from different solvents of S. marianum possess significant antioxidant activity, as well as antimicrobial effects which are all concentration dependent. Thus, the result suggests that methanolic/water leaf extract of S. marianum could serve as a potential source of antioxidants and can be explored as a therapeutic agent in free radical induced diseases. Keywords: Antimicrobial, Antioxidant, Nanoparticles, Phytochemicals, Silybum marianum
Materyal türü: Thesis
Mevcut
Materyal türü Geçerli Kütüphane Koleksiyon Yer Numarası Durum Notlar İade tarihi Barkod Materyal Ayırtmaları
Thesis Thesis CIU LIBRARY Tez Koleksiyonu Tez Koleksiyonu YL 3141 U54 2023 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Pharmacognosy Department T3522
Suppl. CD Suppl. CD CIU LIBRARY Depo YL 3141 U54 2023 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Pharmacognosy Department CDT3522
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Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department

Includes bibliography (sheets 74-84)

ABSTRACT
Silybum marianum is a therapeutic plant belonging to the family Asteraceae. It
contains silymarin, as its principal component, and has been reported to demonstrate
hepatoprotective activity. The study was aimed to investigate the phytochemical
constituents, synthesis of nanoparticles, antioxidant activity and antimicrobial effects
of the leaf extract. The physiochemical analysis revealed moisture content, total ash,
acid insoluble, alcohol, water, and petroleum ether soluble extraction of 13.9 %, 1.35
%, 0.95 %, 5.8 %, 7.1 % and 0.8 % respectively. The chemical profile was evaluated
by Gas Chromatography-Mass Spectrometry (GC-MS) assay and qualitative
phytochemical. Extracts were synthesized from AgNO3 and characterized via FT-IR,
U.V and XRD. The antioxidant activity was determined using DPPH, H2O2 and βcarotene assays. Similarly, all extracts expressed significant DPPH inhibition (IC50) of
5.1, 5.0, 4.7, 2.3, 4.3 and 1.1 (ug/mL), H2O2 of 1.3, 2.2, 4.7, 5.6, 4.0 and 1.0 (ug/mL)
from the methanol/water extract, nanoparticles, n-Hexane, ETOAC, n-Butanol and
Ascorbic acid (standard) respectively. The extract exerted scavenging activity on βcarotene with a significant IC50 inhibition of 1.5, 0.8, 5.2, 0.8, 2.7, 4.8 at 0 hour and
1.5, 0.8, 5.2, 0.8, 2.7 and 4.8 (ug/mL) after 2 hours water bath incubation at 50°C in
the order of the extracts above. Additionally, nanoparticles and plant extracts
significantly (p<0.0001) inhibited the growth of bacteria with mean zone of inhibition
ranging 7-13 mm and 4-11 mm. This study demonstrated that extracts from different
solvents of S. marianum possess significant antioxidant activity, as well as
antimicrobial effects which are all concentration dependent. Thus, the result suggests
that methanolic/water leaf extract of S. marianum could serve as a potential source of
antioxidants and can be explored as a therapeutic agent in free radical induced
diseases.
Keywords: Antimicrobial, Antioxidant, Nanoparticles, Phytochemicals, Silybum
marianum

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