IN-VITRO ASSESSMENT OF NANOPARTICLES AS DRUG DELIVERY FOR THE EVALUATION OF ANTICANCER, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES: CASE; BIOGENIC ZINC OXIDE NANOPARTICLES, MDA-MB 231 CELLS / MAUREEN BAKOR TABE; SUPERVISOR: ASST. PROF. DR. ÖVGÜ İŞBİLEN

Yazar: Katkıda bulunan(lar):Dil: İngilizce 2023İçerik türü:
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Konu(lar): Tez notu: Thesis (MSc) - Cyprus International University Institute of Graduate Studies and Research Bioengineering Department Özet: ABSTRACT Nanotechnology had long been in existence but was yet to be fully understood. In recent years it gained attention in the light of biomedical research especially owing to the better understanding of the nanoscale particles. In which case biological nanoscale particles have gained more attention compared to chemical and physical methods. For this study, biogenic ZnO NPs were synthesized using zinc nitrate tetrahydrate and ethanolic extract of Z. officinale roots. Zn metals are known for their excellent biological potential and minimal level of toxicity. On the other hand, Z. officinale has long been accounted for its good medicinal value which was confirmed by the GCMS analysis revealing the presence of essential constituents. The biogenic ZnO NPs and zinc nitrate tetrahydrate were analysed using UV-Vis spectroscopy, zeta sizer analyser, FTIR, XRD and SEM. Furthermore, the antimicrobial potential was examined against; B. cereus ATCC 7064, Meticillin Resistant S. aureus ATCC 6538P, E. faecalis ATCC 29212, L. monocytogenes, Uropathogenic Escherishia coli UTI-89, P. aeruginosa ATCC 27853, L. pneumophila ATCC 33152, Carbapenem Resistant Klebsiella Pneumoniae (CRKP-239), Carbapenem Resistant Klebsiella Pneumoniae (CRKP 682) and Carbapenem Resistant Klebsiella Pneumoniae (CRKP-692). Both the antioxidant and cytotoxicity potential were investigated. XRD expressed proper crystalline morphology. The zeta sizer recorded a nanosize of 67±89 which tells its nanoscale property. ZnO NPs exhibited a well-defined zone of inhibition and low MIC values between 50-3.25 µg/mL. Biogenic ZnO NPs (42.42±43.95 at 12.5±100 µg/mL respectively) and Z. officinale extract (32.75±40.22 at 12.5±100 µg/mL respectively) revealed antioxidant potential and moderate cytotoxicity activity against MDA-MB 231 cells. Thus, ZnO NPs and Z. officinale extract demonstrated a wide range of significant importance in biomedical research. Keywords: Antimicrobial, Antioxidant, Cytotoxicity, ZnO NPs, Z. officinale.
Materyal türü: Thesis
Mevcut
Materyal türü Geçerli Kütüphane Koleksiyon Yer Numarası Durum Notlar İade tarihi Barkod Materyal Ayırtmaları
Thesis Thesis CIU LIBRARY Depo Tez Koleksiyonu YL 3238 T33 2023 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Bioengineering Department T3632
Suppl. CD Suppl. CD CIU LIBRARY Görsel İşitsel Tez Koleksiyonu YL 3238 T33 2023 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Bioengineering Department CDT3632
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Thesis (MSc) - Cyprus International University Institute of Graduate Studies and Research Bioengineering Department

Includes References (sheets 104-116)

ABSTRACT
Nanotechnology had long been in existence but was yet to be fully understood. In recent years it gained attention in the light of biomedical research especially owing to the better understanding of the nanoscale particles. In which case biological nanoscale particles have gained more attention compared to chemical and physical methods. For this study, biogenic ZnO NPs were synthesized using zinc nitrate tetrahydrate and ethanolic extract of Z. officinale roots. Zn metals are known for their excellent biological potential and minimal level of toxicity. On the other hand, Z. officinale has long been accounted for its good medicinal value which was confirmed by the GCMS analysis revealing the presence of essential constituents. The biogenic ZnO NPs and zinc nitrate tetrahydrate were analysed using UV-Vis spectroscopy, zeta sizer analyser, FTIR, XRD and SEM. Furthermore, the antimicrobial potential was examined against; B. cereus ATCC 7064, Meticillin Resistant S. aureus ATCC 6538P, E. faecalis ATCC 29212, L. monocytogenes, Uropathogenic Escherishia coli UTI-89, P. aeruginosa ATCC 27853, L. pneumophila ATCC 33152, Carbapenem Resistant Klebsiella Pneumoniae (CRKP-239), Carbapenem Resistant Klebsiella Pneumoniae (CRKP 682) and Carbapenem Resistant Klebsiella Pneumoniae (CRKP-692). Both the antioxidant and cytotoxicity potential were investigated. XRD expressed proper crystalline morphology. The zeta sizer recorded a nanosize of 67±89 which tells its nanoscale property. ZnO NPs exhibited a well-defined zone of inhibition and low MIC values between 50-3.25 µg/mL. Biogenic ZnO NPs (42.42±43.95 at 12.5±100 µg/mL respectively) and Z. officinale extract (32.75±40.22 at 12.5±100 µg/mL respectively) revealed antioxidant potential and moderate cytotoxicity activity against MDA-MB
231 cells. Thus, ZnO NPs and Z. officinale extract demonstrated a wide range of significant importance in biomedical research.
Keywords: Antimicrobial, Antioxidant, Cytotoxicity, ZnO NPs, Z. officinale.

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