Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering

Wounds healing is a sophisticated biological process that involves numerous cellular and molecular activities. In recent years, nanotechnology has emerged as an appealing option for enhancing wound treatment methods. This work investigates the potential of curcumin-loaded zinc oxide nanoparticles (ZnO NPs) as an innovative therapeutic approach to enhance wound healing at the cellular level.
The nanoparticles were synthesized using a green synthesis approach to ensure a safer method of production. Curcumin, an anti-inflammatory and antioxidant compound, was used to load the zinc oxide nanoparticles. The nanoparticles were characterized using Ultraviolet-Visible (UV-VIS) spectrophotometer, Fourier Transform Infrared (F.T-I.R) and X-Ray Diffraction (X.R.D). A zeta size analyzer was used to determine the size and charge of the biosynthesized zinc oxide nanoparticles, revealing that the biologically synthesized nanoparticle had an average size of nm.
The incorporation of curcumin-loaded ZnO NPs with DMEM was investigated as a treatment for the wound healing assay to assess their potential in promoting wound closure. Curcumin and zinc oxide work together to enhance wound closure, making these nanoparticles a promising option for developing novel wound care therapies. Further research into the in-vivo effectiveness and safety of these nanoparticles will be critical to fully addressing their therapeutic potential.