Removal of Bisphenol a from Aqueous solution using cone of Pinus Resinosa as a novel biosorbent Desire Kemena Awoh; Supervisor: Hatice Erkurt

Yazar: Katkıda bulunan(lar):Dil: İngilizce Yayın ayrıntıları:Nicosia Cyprus International University 2013Tanım: XI, 65 p. table 30.5 cm CDİçerik türü:
  • text
Ortam türü:
  • unmediated
Taşıyıcı türü:
  • volume
Konu(lar):
Eksik içerik
1 CHAPTER 1
1 INTRODUCTION
1 Endocrine System
1 Endocrine Disruptive Compounds (EDCs)
3 Health effect of EDCs
4 Mode of exposure to EDCs
4 Description of Some Environmental EDCs
5 Environmental Exposure and Health Implications
6 Bisphenol A
8 Common Uses of BPA
8 Environmental Fate of BPA
10 Health effect of BPA
10 Government regulation and discrepancies on BPA
11 Research Objective and Motivation
12 CHAPTER 2
12 LITERATURE REVIEW
13 Cones of Pinus resinosa(Pine Cones)
15 Biosorption
15 Bisorbent
16 Source of Biomas for Biosorption
16 Characteristics of Biosorbent
16 Bisphenol A
17 Enzymatic Treament
17 ENZYMES
21 CHAPTER 3
21 ANALYTICAL INSTRUMENTS
21 High Performance Liquid Chromatography (HPLC)
21 Components of the HPLC Machine
22 How the HPLC Operates
24 Chromatographic view for BPA
26 Concentration Measurements
27 pH balance
27 Oven
27 Shaker
28 Centrifuge
29 Micro Filter
29 MATERIALS AND METHODS
29 MATERIALS
29 Bisorbents Preparation
29 Chemicals
30 METHODS
30 Effects of pH
30 Effect of Temperature
30 Effects of Bio sorbent Dosage
31 Adsorption Isotherm
32 Enzymatic Study
32 CHAPTER 5
34 RESULTS AND DISCUSSION
34 Effects of pH
36 Effects of Bio sorbent Dosage
45 Effect of Temperature
47 Effect of initial concentration on BPA Sorption
48 Adsorption Isotherm
52 Percentage removal of BPA by Laccase Enzymes
54 Result Comparison
56 CHAPTER 6
56 CONCLUSIONS
57 REFERENCES
Özet: 'Abstract The use of biosorption technology in the removal of Endocrine Disruptive compound has being extensively studied and this work focuses on the use of cone of Pinus resinosa (as a adsorbent) for the removal of Bisphenol A (BPA) from a wastewater solution. Biosorption is the use of biological materials as sorbent using their physic-chemical pathways. This work investigates the removal of BPA under the following parameters, the effect of temperature, pH, initial BPA concentration, temperatures and biomass dosage. The maximun adsorption capacity of pine cone powder for BPA removal was (0.155 mg/g), the effective pH was 5.0. The percentage removal of pine was found to be at maximuim at 60 0C after 30mimutes and was (65.5 %). The study was carried out with different temperature ranges (40 0C, 50 0C, 60 0C), and different initial concentrations of 0.1ppm, 0.15ppm, 0.25ppm, and 0.5ppm. The biomass dosages are (0.3g, 0.2g, 0.1g and 0.05g). The result also shows that there was increase in the percentage removal as the biomasses increases but the sorption capacity was found to big high for 0.05g of pine cone powder. The Langmuir, Freundlich and Temkin isotherm models were calculated and the results fits well with the Temkin isotherm models for all three temperatures and it fits well for Freundlich isotherm models at 40 0C but did not fit the Langmuir isotherm models for all three temperatures. The R2 for Temkin isotherm models are (0.9233, 0.914, and 0.9833) for 40 0C, 50 0C, 60 0C, respectively. The results of the percentage biosorption removal were compared with the enzymatic removal with Laccase enzyme. The experiments was carried out with pH 4.0 C6H8O7 buffer solution and result for 4hours with 0.1ppm (23.66%) and 0.5ppm (24.38%) were compared with that of resinosa percentage removal. Keywords: Pine cone; Bisphenol A, Biosorption. Özet Endokrin bozucu bileşiklerin gideriminde biyosorpsiyon yöntemi yaygın olarak kullanılmaktadır. Bu çalışmada Bisfenol A'nı giderimi için Pinus resinosa kozalak tozu kullanılmıştır. Çalışmada, biyolojik materyaller sorbent olarak kullanılmıştır. Çalışmada, sıcaklık, pH, başlangıç BPA derişimi ve biyokütle derişiminin biyosorpsiyon üzerine etkisi incelenmiştir. Kozalak tozunun maksimum adsorpsiyon kapsitesi pH 5'de 0.155 mg/g olarak belirlenmiştir. Çam kozalağını maksimum BPA giderim verimi 30. dakikada, 60 0C de 65.5% olarak saptanmıştır. Çalışma, farklı sıcaklıklarda (40 0C, 50 0C, 60 0C), farklı BPA derişimlerinde (0.1ppm, 0.15ppm, 0.25ppm, and 0.5ppm) ve farklı biyokütle miktarları (0.3g, 0.2g, 0.1g and 0.05g) kullanılarak sürdürülmüştür. Çalışma bulguları, biyokütle miktarı arttıkça BPA giderim veriminin arttığını, ancak biyosorpsiyon kapasitesinin arttığını; 0.05 g biyokütle ile en yüksek biyosorpsiyon kapasitesi saptanmıştır.Deneysel verilerin Langmuir, Freundlich and Temkin izoerm modellerine uygunlukları araştırılmış, farklı sıcaklıklarda elde edilen verilerin Temkin izoterm modeline uyum gösterdiği, 40 0C de elde edilen verilerin Freundlich modeline de uyum gösterdiği ancak farklı sıcaklıklarda elde edilen verilerin Langmuir modeline uyum göstermediği sonucuna varılmıştır. Temkin izoterm modeli için 40 0C, 50 0C, 60 0C'de elde edilen R2 değerleri sırası ile 0.9233, 0.9804, and 0.999 olarak belirlenmiştir. BPA'nın biyosorpsiyonla giderim verimi ve lakkaz enzimi ile enzimatik giderim verimi karşılaştırılmıştır. Enzimatik BPA giderim çalışmaları pH 4 sodyum sitrat tamponu kullanılarak sürdürülmüş ve 0.1 ppm ve 0.5 ppm BPA için 30 dakikalık deney sonucu elde edilen veriler biyosorpsiyon verileri ile karşılaştırılmıştır. Anahtar Kelimeler: Pinus resinosa; Çam kozalağı; Bisfenol A; Biosorpsiyon.'
Materyal türü: Thesis

Includes CD

Includes references (57-64 p.)

'Abstract The use of biosorption technology in the removal of Endocrine Disruptive compound has being extensively studied and this work focuses on the use of cone of Pinus resinosa (as a adsorbent) for the removal of Bisphenol A (BPA) from a wastewater solution. Biosorption is the use of biological materials as sorbent using their physic-chemical pathways. This work investigates the removal of BPA under the following parameters, the effect of temperature, pH, initial BPA concentration, temperatures and biomass dosage. The maximun adsorption capacity of pine cone powder for BPA removal was (0.155 mg/g), the effective pH was 5.0. The percentage removal of pine was found to be at maximuim at 60 0C after 30mimutes and was (65.5 %). The study was carried out with different temperature ranges (40 0C, 50 0C, 60 0C), and different initial concentrations of 0.1ppm, 0.15ppm, 0.25ppm, and 0.5ppm. The biomass dosages are (0.3g, 0.2g, 0.1g and 0.05g). The result also shows that there was increase in the percentage removal as the biomasses increases but the sorption capacity was found to big high for 0.05g of pine cone powder. The Langmuir, Freundlich and Temkin isotherm models were calculated and the results fits well with the Temkin isotherm models for all three temperatures and it fits well for Freundlich isotherm models at 40 0C but did not fit the Langmuir isotherm models for all three temperatures. The R2 for Temkin isotherm models are (0.9233, 0.914, and 0.9833) for 40 0C, 50 0C, 60 0C, respectively. The results of the percentage biosorption removal were compared with the enzymatic removal with Laccase enzyme. The experiments was carried out with pH 4.0 C6H8O7 buffer solution and result for 4hours with 0.1ppm (23.66%) and 0.5ppm (24.38%) were compared with that of resinosa percentage removal. Keywords: Pine cone; Bisphenol A, Biosorption. Özet Endokrin bozucu bileşiklerin gideriminde biyosorpsiyon yöntemi yaygın olarak kullanılmaktadır. Bu çalışmada Bisfenol A'nı giderimi için Pinus resinosa kozalak tozu kullanılmıştır. Çalışmada, biyolojik materyaller sorbent olarak kullanılmıştır. Çalışmada, sıcaklık, pH, başlangıç BPA derişimi ve biyokütle derişiminin biyosorpsiyon üzerine etkisi incelenmiştir. Kozalak tozunun maksimum adsorpsiyon kapsitesi pH 5'de 0.155 mg/g olarak belirlenmiştir. Çam kozalağını maksimum BPA giderim verimi 30. dakikada, 60 0C de 65.5% olarak saptanmıştır. Çalışma, farklı sıcaklıklarda (40 0C, 50 0C, 60 0C), farklı BPA derişimlerinde (0.1ppm, 0.15ppm, 0.25ppm, and 0.5ppm) ve farklı biyokütle miktarları (0.3g, 0.2g, 0.1g and 0.05g) kullanılarak sürdürülmüştür. Çalışma bulguları, biyokütle miktarı arttıkça BPA giderim veriminin arttığını, ancak biyosorpsiyon kapasitesinin arttığını; 0.05 g biyokütle ile en yüksek biyosorpsiyon kapasitesi saptanmıştır.Deneysel verilerin Langmuir, Freundlich and Temkin izoerm modellerine uygunlukları araştırılmış, farklı sıcaklıklarda elde edilen verilerin Temkin izoterm modeline uyum gösterdiği, 40 0C de elde edilen verilerin Freundlich modeline de uyum gösterdiği ancak farklı sıcaklıklarda elde edilen verilerin Langmuir modeline uyum göstermediği sonucuna varılmıştır. Temkin izoterm modeli için 40 0C, 50 0C, 60 0C'de elde edilen R2 değerleri sırası ile 0.9233, 0.9804, and 0.999 olarak belirlenmiştir. BPA'nın biyosorpsiyonla giderim verimi ve lakkaz enzimi ile enzimatik giderim verimi karşılaştırılmıştır. Enzimatik BPA giderim çalışmaları pH 4 sodyum sitrat tamponu kullanılarak sürdürülmüş ve 0.1 ppm ve 0.5 ppm BPA için 30 dakikalık deney sonucu elde edilen veriler biyosorpsiyon verileri ile karşılaştırılmıştır. Anahtar Kelimeler: Pinus resinosa; Çam kozalağı; Bisfenol A; Biosorpsiyon.'

1 CHAPTER 1

1 INTRODUCTION

1 Endocrine System

1 Endocrine Disruptive Compounds (EDCs)

3 Health effect of EDCs

4 Mode of exposure to EDCs

4 Description of Some Environmental EDCs

5 Environmental Exposure and Health Implications

6 Bisphenol A

8 Common Uses of BPA

8 Environmental Fate of BPA

10 Health effect of BPA

10 Government regulation and discrepancies on BPA

11 Research Objective and Motivation

12 CHAPTER 2

12 LITERATURE REVIEW

13 Cones of Pinus resinosa(Pine Cones)

15 Biosorption

15 Bisorbent

16 Source of Biomas for Biosorption

16 Characteristics of Biosorbent

16 Bisphenol A

17 Enzymatic Treament

17 ENZYMES

21 CHAPTER 3

21 ANALYTICAL INSTRUMENTS

21 High Performance Liquid Chromatography (HPLC)

21 Components of the HPLC Machine

22 How the HPLC Operates

24 Chromatographic view for BPA

26 Concentration Measurements

27 pH balance

27 Oven

27 Shaker

28 Centrifuge

29 Micro Filter

29 MATERIALS AND METHODS

29 MATERIALS

29 Bisorbents Preparation

29 Chemicals

30 METHODS

30 Effects of pH

30 Effect of Temperature

30 Effects of Bio sorbent Dosage

31 Adsorption Isotherm

32 Enzymatic Study

32 CHAPTER 5

34 RESULTS AND DISCUSSION

34 Effects of pH

36 Effects of Bio sorbent Dosage

45 Effect of Temperature

47 Effect of initial concentration on BPA Sorption

48 Adsorption Isotherm

52 Percentage removal of BPA by Laccase Enzymes

54 Result Comparison

56 CHAPTER 6

56 CONCLUSIONS

57 REFERENCES

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