SYNTHESIS AND CHARACTERIZATION OF ZINC OXIDE NANOPARTICLES VIA PLANT EXRTACT OF AGAVE AMERICANA /
ANTHONY JUNIOR ADEJI; SUPERVISOR: PROF. DR. HATICE ERKURT
- ix, 59 sheets; 31 cm. Includes CD
Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
Includes bibliography (sheets 52-59)
ABSTRACT The fabrication of nanomaterials can be done using a variety of plant resources thanks to green technology, which also avoids the use of any processes that could be considered destructive. This research concentrates on an effective, simple, and eco friendly creation of ZnO nanoparticles using zinc sulfate heptahydrate and the bio components of dry agave powder extract (Agave americana). The characterization of the nanoparticles was done by using the analysis of Ultraviolet-Visible (UV-VIS) spectrophotometer, Fourier Transform Infrared (F.T-I.R), X-Ray Diffraction (X.R.D), and Scanning Electron Microscope (S.E.M). The size of the biosynthesized Zinc oxide nanoparticle was evaluated using the zeta size analyzer, which reveals that the biologically synthesized nanoparticle has an average size of 78nm. The presence of a peak between 271 and 289nm, which was observed via the uv-vis absorption is evidence that ZnONPs had been found. The S.E.M study shows a spherical form with a size range of 50 to 98nm while the X.R.D patterns indicated that the wurtzite structure is in the hexagonal phase. The existence of OH, C-H, C-O, and C-N stretching was demonstrated by the F.T-IR analysis. The use of a quantitative analysis of the aqueous solution of the leaf extract of agave americana enabled the detection of the presence of phytochemical compounds, and bioactive active compounds such as saponins, tannins, and flavonoids were present. According to the findings of the antioxidant activity, the aqueous plant extract and ZnONPs showed a scavenging value of 98% and 94% respectively, when the D.P.P.H assay was carried out. On the antibacterial experimentation, the bactericidal effect of both gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria were evaluated using the bio synthesized ZnONPs. B. subtilis and E.coli showed a zone of inhibition at 28mm and 5mm respectively. This research, confirmed the presence of pharmacologically active chemicals in the green synthesized ZnO nanoparticles which paves the door for their practical implementation in the therapeutic, treatment of industrial effluents and nanotechnology for drug delivery systems. Keywords; Agave americana, Antibacterial, Antioxidant, Characterization, Phytochemicals, Zinc Sulfate Heptahydrate, ZnONPs