BIOSYNTHESIS OF SILVER NANOPARTICLES AND CATHARANTUS ROSES AND THEIR ANTIBACTERIAL EFFICACY /
ANUOLUWAPO MODUPE OYAWALE; SUPERVISOR: ASST. PROF. DR. PWADUBASHIYI COSTON PWAVODI
- x, 52 sheets: charts, photos; 31 cm. Includes CD
Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
Includes bibliography (sheets 47-52)
ABSTRACT The methodology for creating silver nanoparticles (AgNPs) is developed in this paper by reducing Catharanthus roseus plant leaf extract. This research creates synthetic nanoparticles with highly effective antibacterial properties that are controlled by the manufacturing method. Smaller size, appropriate shape, evenly distribution, and the creation of functional groups are some of these characteristics by enhancing the parameters used in the reduction procedure. C. roseus leaf extract and AgNO3 were used to undertake the biosynthesis of AgNPs. Silver nitrate of 0.6 g was mixed with distilled water of 500 ml, the ideal conditions and concentration for the creation of nanoparticles were created. A magnetic stirrer was used to agitate the liquid for 5 minutes. After that, Catharanthus roseus extracts were added carefully until the colour changes from white to brownish occurred, showing the presence of synthesized AgNps with Catharanthus roseus. After then, the mixture was stirred for 24 hours. The samples were characterized using a variety of methods, including UV-Vis spectrophotometer analysis, Fourier transforms infrared, X-ray diffraction spectroscopy and Scanning electron microscopy. The size of 88 nm, spherical morphology, functional group, absorbance rate, and crystallinity were all validated by the findings of the characterization process. In the current study, zone of inhibition methods was done to assess the anti-bacterial property of the synthesized silver nanoparticle (AgNPs) against two distinct bacteria at doses of AgNPs (10 g/ml) alone as well as in combination with the antibiotic gentamicin. Lastly, antioxidant activity (DPPH radical scavenging method) was also assessed. According to the findings, bacterial growth is inhibited by AgNPs at high concentrations. Additionally, against bacteria that are resistant to antibiotics, AgNPs had a substantially better antibacterial effect when combined with gentamicin. A practical and environmentally friendly approach for creating silver nanoparticles has been devised utilizing Cantharantus roseus leaf extract. In the near future, the fields of water treatment, biomedicine, and nanotechnology may all benefit from the use of synthesized AgNPs. Keywords; Antimicrobial activity, Antioxidant, Biosynthesis, Catharanthus roseus, Characterization, Silver nanoparticles.