TY  - BOOK
AU  - Ukochovwera,Oghenetejeri
AU  - Pwavodi,Pwadubabashiyi Coston
TI  - GREEN SYNTHESIS OF IRON NANOPRTICLES USING  THE LEAF EXTRACT OF LAURUS NOBILIS L. AND ITS  ASSESMENT OF ANTIBACTERIAL AND  ANTIOXIDANT ACTIVITIES
PY  - 2023///
KW  - Antibacterial agents
KW  - Dissertations, Academic
KW  - Antioxidants
KW  - Laurus nobilis
KW  - Nanotechnology
N1  - Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department; Includes bibliography (sheets 51-58)
N2  - ABSTRACT
The synthesis of nanoparticles using biomaterials is known as green nanotechnology, and it aims 
to promote environmentally sound and long-lasting technologies. This study focuses on the 
straightforward and sustainable production of iron sulphate nanoparticles using an aqueous 
extract of Laurus nobilis L. leaves. X-ray diffraction analysis (XRD), Fourier transform infrared 
(FT-IR), and ultraviolet-visible (UV-visible) spectroscopy were used to characterize the 
nanoparticles. The outcomes demonstrated that the nanoparticles had a crystalline structure, 
were mostly spherical, and had only a small number of hexagonal particles. Zeta analysis 
determined that the average size of the nanoparticles was 60.0 nm. The extraction by maceration 
yield was 10.07% of the total yield. Additionally, six different bacterial species were used to 
examine the nanoparticles' antimicrobial effectiveness. The nanoparticles created using this 
green manufacturing approach showed promising antioxidant and antibacterial properties. 
Phytochemical analysis revealed the presence of tannins, flavonoids, saponins, alkaloids, and 
steroids, all of which are important in transforming iron sulfate into iron nanoparticles with 
antibacterial and antioxidant properties. With inhibition zones ranging from 15 to 25 mm, the 
extracts, and iron nanoparticles showed significant antibacterial activity against Escherichia coli, 
Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa, 
Listeria pneumonia. Using the MIC (minimum inhibitory concentration) assays, the 
effectiveness of Laurus nobilis extracts against the above-mentioned bacterial strains was 
assessed. MIC values for L. nobilis extracts were 0.201 0.01 mg/mL for L. pneumophila, 0.602 
0.15 mg/mL for S. aureus, 0.654 0.04 mg/mL for E. coli, 0.51 0.02 mg/mL for E. faecalis, and 
0.38 0.05 mg/mL for P. aeruginosa. Additionally, all of the examined microbes were resistant 
to the antibacterial effects of gentamycin and iron nanoparticle concentrations.
Keywords: Antibacterial, Antioxidant, Green Synthesis, Laurus nobilis
Nanotechnology, Phytochemical Screening
ER  -