GREEN SYNTHESIS OF ZINC OXIDE NANOPARTICLES USING ETHANOLIC LEAF EXTRACT OF OLEA EUROPAEA L. AND IT’S IN VITRO EVALUATION ON MDA-MB 231 CANCER CELLS LINES, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIY /
ISMAILA CEESAY SUPERVISOR ASST. PROF. DR. PWADUBASHIYI COSTON PWAVO DI
- xv, 106 sheets; 31 cm. 1 CD-ROM
Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
Includes bibliography (sheets 96-106)
ABSTRACT Most women get breast cancer, 1.4 million cases are diagnosed annually worldwide. Zinc oxide nanoparticle biosynthesis of olive leaves is a popular traditional leave study. This study was aim to synthesised and characterised zinc oxide nanoparticles using Olea europaea L to test their cytotoxic impact on breast cancer cell lines MDAMB 231, antioxidant, and antibacterial properties on gram-negative and gram-positive bacteria. The GC-MS analysis shows that there are some bioactive compound presents in Olea europaea L and the UV spectra analysis indicated the spectrum with the peaks of 313, 285, 257, 227 and 201 nm with absorption respectively. The zeta size has shown a size of 86.14 nm with a charge of -12.64 mV X-RAY Diffraction (XRD) and the Fourier Transform Infrared Spectrophotometer (FTIR) has been used. The antioxidant result shows an antioxidant activity of the ZnO NPs and Zn against the free radicals while higher activity was recorded in Olea europaea L. The ZnO NPs, Zn and Olea europaea L against the six bacterial has shown a zone of inhibition for the following bacterial P. aeruginosa 29, E. coli 12, S. aureus 16, B. cereus 8, while the two bacterial E faecalis 0, Legionella pneumophila 0, has not indicated any zone of inhibition which has been found by other researcher of similar result E Faecalis, legionella pnenumophila. DMEM-grown MDA MB 231 cells for positive control were 100% viable. After 24 hours of treatment, cell was reduced to 80%. The higher dose reduced cells from 100% to 80% and after 48-hour of incubation there was 68% of effects of ZnO NPs. Positive control of MDA MB 231 and DMEM as a media shown 100% viability in 24-hour at the beginning, Minor influence seen in 24-hour cell death of 80%, while the positive control still maintains the cell viability at 100%. ZnO nanoparticles activity caused shrank, aggregated, deformed, adhered, and proliferated slower MDA MB 231 breast cancer cells as evident of Apoptosis and shrinkage indicate cell death. Keywords: Antibacterial, Anticancer Antioxidant, Breast Cancer and Olea europaea L