GREEN SYNTHESIS AND CHARACTERIZATION OF BRASSICA RAPA SUBSP, NARINOSA SILVER NANOPARTICLES FOR ANTIMICROBIAL, ANTIOXIDANT APPLICATION AND CATALYTIC DEGARDATION OF SELECTED DYES /
ADAM SALEH HASSAN ; SUPERVISOR, PROF. DR. NUR PAŞAOĞLULARI AYDINLIK
- 69 sheets ; 30 cm +1 CD ROM
Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Chemistry
The leaf extract of Brassica rapa subsp. narinosa (tatsoi) is used in this study to investigate the green production of silver nanoparticles (AgNPs) and assess their antibacterial, antioxidant, and dye-catalytic degradation properties. Phytochemicals were used in the fast synthesis process as reducing and stabilizing agents; a color shift to dark brown signified successful creation. The presence and characteristics of the nanoparticles, such as a prominent UV absorption peak at 460 nm and a zeta potential of -21.85 mV, indicating stability, were confirmed by characterization techniques such as UV-visible spectroscopy, Fourier Transform-Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Zeta sizer, and X-ray diffraction (XRD). Under specified conditions, the produced AgNPs showed substantial catalytic activity, reaching 60% degradation of methylene blue and 90% degradation of safranin. The DPPH assay was also used to evaluate the antioxidant activity. The results showed that the plant extract had higher antioxidant activity than the nanoparticles, with IC50 values of 8.409 μg/ml and 13.40 μg/ml, respectively. Gram-positive Bacillus subtilis (B. subtilis) and Gram-negative Escherichia coli (E.coli) were efficiently stopped from growing by AgNPs, according to antimicrobial tests, but the plant extract by itself showed no antibacterial action. This work demonstrates the possibility of ecologically friendly nanoparticle production for useful uses in antioxidant therapy, antibacterial treatments, and dye pollution cleanup in the environment.