Removal of Bisphenol a from Aqueous solution using cone of Pinus Resinosa as a novel biosorbent
Desire Kemena Awoh; Supervisor: Hatice Erkurt
- Nicosia Cyprus International University 2013
- XI, 65 p. table 30.5 cm CD
Includes CD
Includes references (57-64 p.)
CHAPTER 1 1 INTRODUCTION 1 Endocrine System 1 Endocrine Disruptive Compounds (EDCs) 1 Health effect of EDCs 3 Mode of exposure to EDCs 4 Description of Some Environmental EDCs 4 Environmental Exposure and Health Implications 5 Bisphenol A 6 Common Uses of BPA 8 Environmental Fate of BPA 8 Health effect of BPA 10 Government regulation and discrepancies on BPA 10 Research Objective and Motivation 11 CHAPTER 2 12 LITERATURE REVIEW 12 Cones of Pinus resinosa(Pine Cones) 13 Biosorption 15 Bisorbent 15 Source of Biomas for Biosorption 16 Characteristics of Biosorbent 16 Bisphenol A 16 Enzymatic Treament 17 ENZYMES 17 CHAPTER 3 21 ANALYTICAL INSTRUMENTS 21 High Performance Liquid Chromatography (HPLC) 21 Components of the HPLC Machine 21 How the HPLC Operates 22 Chromatographic view for BPA 24 Concentration Measurements 26 pH balance 27 Oven 27 Shaker 27 Centrifuge 28 Micro Filter 29 MATERIALS AND METHODS 29 MATERIALS 29 Bisorbents Preparation 29 Chemicals 29 METHODS 30 Effects of pH 30 Effect of Temperature 30 Effects of Bio sorbent Dosage 30 Adsorption Isotherm 31 Enzymatic Study 32 CHAPTER 5 32 RESULTS AND DISCUSSION 34 Effects of pH 34 Effects of Bio sorbent Dosage 36 Effect of Temperature 45 Effect of initial concentration on BPA Sorption 47 Adsorption Isotherm 48 Percentage removal of BPA by Laccase Enzymes 52 Result Comparison 54 CHAPTER 6 56 CONCLUSIONS 56 REFERENCES 57
'Abstract The use of biosorption technology in the removal of Endocrine Disruptive compound has being extensively studied and this work focuses on the use of cone of Pinus resinosa (as a adsorbent) for the removal of Bisphenol A (BPA) from a wastewater solution. Biosorption is the use of biological materials as sorbent using their physic-chemical pathways. This work investigates the removal of BPA under the following parameters, the effect of temperature, pH, initial BPA concentration, temperatures and biomass dosage. The maximun adsorption capacity of pine cone powder for BPA removal was (0.155 mg/g), the effective pH was 5.0. The percentage removal of pine was found to be at maximuim at 60 0C after 30mimutes and was (65.5 %). The study was carried out with different temperature ranges (40 0C, 50 0C, 60 0C), and different initial concentrations of 0.1ppm, 0.15ppm, 0.25ppm, and 0.5ppm. The biomass dosages are (0.3g, 0.2g, 0.1g and 0.05g). The result also shows that there was increase in the percentage removal as the biomasses increases but the sorption capacity was found to big high for 0.05g of pine cone powder. The Langmuir, Freundlich and Temkin isotherm models were calculated and the results fits well with the Temkin isotherm models for all three temperatures and it fits well for Freundlich isotherm models at 40 0C but did not fit the Langmuir isotherm models for all three temperatures. The R2 for Temkin isotherm models are (0.9233, 0.914, and 0.9833) for 40 0C, 50 0C, 60 0C, respectively. The results of the percentage biosorption removal were compared with the enzymatic removal with Laccase enzyme. The experiments was carried out with pH 4.0 C6H8O7 buffer solution and result for 4hours with 0.1ppm (23.66%) and 0.5ppm (24.38%) were compared with that of resinosa percentage removal. Keywords: Pine cone; Bisphenol A, Biosorption. Özet Endokrin bozucu bileşiklerin gideriminde biyosorpsiyon yöntemi yaygın olarak kullanılmaktadır. Bu çalışmada Bisfenol A'nı giderimi için Pinus resinosa kozalak tozu kullanılmıştır. Çalışmada, biyolojik materyaller sorbent olarak kullanılmıştır. Çalışmada, sıcaklık, pH, başlangıç BPA derişimi ve biyokütle derişiminin biyosorpsiyon üzerine etkisi incelenmiştir. Kozalak tozunun maksimum adsorpsiyon kapsitesi pH 5'de 0.155 mg/g olarak belirlenmiştir. Çam kozalağını maksimum BPA giderim verimi 30. dakikada, 60 0C de 65.5% olarak saptanmıştır. Çalışma, farklı sıcaklıklarda (40 0C, 50 0C, 60 0C), farklı BPA derişimlerinde (0.1ppm, 0.15ppm, 0.25ppm, and 0.5ppm) ve farklı biyokütle miktarları (0.3g, 0.2g, 0.1g and 0.05g) kullanılarak sürdürülmüştür. Çalışma bulguları, biyokütle miktarı arttıkça BPA giderim veriminin arttığını, ancak biyosorpsiyon kapasitesinin arttığını; 0.05 g biyokütle ile en yüksek biyosorpsiyon kapasitesi saptanmıştır.Deneysel verilerin Langmuir, Freundlich and Temkin izoerm modellerine uygunlukları araştırılmış, farklı sıcaklıklarda elde edilen verilerin Temkin izoterm modeline uyum gösterdiği, 40 0C de elde edilen verilerin Freundlich modeline de uyum gösterdiği ancak farklı sıcaklıklarda elde edilen verilerin Langmuir modeline uyum göstermediği sonucuna varılmıştır. Temkin izoterm modeli için 40 0C, 50 0C, 60 0C'de elde edilen R2 değerleri sırası ile 0.9233, 0.9804, and 0.999 olarak belirlenmiştir. BPA'nın biyosorpsiyonla giderim verimi ve lakkaz enzimi ile enzimatik giderim verimi karşılaştırılmıştır. Enzimatik BPA giderim çalışmaları pH 4 sodyum sitrat tamponu kullanılarak sürdürülmüş ve 0.1 ppm ve 0.5 ppm BPA için 30 dakikalık deney sonucu elde edilen veriler biyosorpsiyon verileri ile karşılaştırılmıştır. Anahtar Kelimeler: Pinus resinosa; Çam kozalağı; Bisfenol A; Biosorpsiyon.'