000			03169nam a22003017a 4500
003			KOHA
005			20230425113622.0
008			221021d2022 cy ||||| m||| 00| 0 eng d
040			_aCY-NiCIU _beng _cCY-NiCIU _erda
041			_aeng
090			_aYL 2564 _bL23 2022
100	1		_aLaaboudi, Ahlam
245	1	0	_aPHYTOCHEMICAL, AND SYNTHESIS OF SILVER NANOPARTICLES FROM CITRUS RETICULATA PEELS AND ITS ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY / _cAHLAM LAABOUDI; SUPERVISOR: ASSY. PROF. DR. EMMANUEL MSHELLA HALILU
264			_c2022
300			_a50 sheets; _c31 cm. _eIncludes CD
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
502			_aThesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department
504			_aIncludes bibliography (sheets 47-50)
520			_aABSTRACT Tangerine (Citrus reticulata L.) is a type of citrus plant. Citrus is a genus of flowering plants in the family Rutaceae and a common name for edible fruits of this genus. Citrus contains phenolic bioactive compounds which confirred a powerful antioxidant, antiinflammatory and anticancer properties.Therefore this research is aimed to evaluate the phytochemical and antioxidant effect of tangerine peels extracts/syilver nanoparticles. The Tangerine peels powder was evalueted for its pharmacognostic properties and then extracted with methanol using maceration and the extract was subjected to the phytochemical studies. The thin layer chromatography (TLC) were conducted using different solvent systems and the Rf values of the separeted component were calculated . The synthesis of Nanoparticles was made using Silver nitrate (AgNO3), and the nanoparticles was charactarized using FT-IR and SEM analysis. The antioxidant activity of the extract were determined using DPPH and Hydrogen peroxide Scavenging Assay (HPSA) using ascorbic acid as standard. The antibacterial activity of the extract and silver nanoparticles was performed using agar diffusion method against Escherichia coli and Staphylococcus aureus. The premilinary phytochemical screening of the extract indicate the presence of tanins,saponins,alkaloids and flavonoids . The free radical scavening activity of the plant extract showed IC50 of 6.17 mg/ml using DPPH, and IC50 of 8.4 mg/ml using HPSA. The ascorbic acid (standard) showed IC50 = 1.929 mg/ml. The Antibacterial test releaved that the tangerine peels plant extract had an antibacteerial activity against the Gram postive Staphylococcus aureus and no activity against Escherichia coli .The results indicated that the extract contain phenolic coumpounds that can scavenge free radicals and inbibit the growth of Gram postive bacteria. Keywords: Amtibacterial, Antioxidant ,Citrus reticulata, DPPH
650		0	_aAntibacterial agents _vDissertations, Academic
650		0	_aAntioxidants _vDissertations, Academic
650		0	_aMandarin orange _vDissertations, Academic
700	1		_aHalilu, Emmanuela Mshellia _esupervisor
942			_2ddc _cTS
999			_c285459 _d285459