000			03401nam a22003017a 4500
003			KOHA
005			20230425090801.0
008			221027d2022 cy ||||| m||| 00| 0 eng d
040			_aCY-NiCIU _beng _cCY-NiCIU _erda
041			_aeng
090			_aYL 2511 _bO46 2022
100	1		_aOmolan, Emmanuel
245	1	0	_aEVALUATION OF CHEMICAL, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF Erythrina senegalensis D.C STEM BARK EXTRACT / _cEMMANUEL OMOLAN; SUPERVISOR: ASST. PROF. DR. EMMANUEL MSHELIA HALILU
264			_c2022
300			_a91 sheets; _c31 cm. _eIncludes CD
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
502			_aThesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department
504			_aIncludes bibliography (sheets 73-91)
520			_aABSTRACT Erythrina senegalensis, the Senegal coral tree is a plant which belongs to the family of Fabaceae and it has been used by traditional healers to treat many ailments and infections in African traditional medicine. There is a growing concern about the scourge of diseases caused by excessive free radicals in the body and lack of standardization of medicinal herbal products. Therefore, this research is aimed at evaluating the chemical, antibacterial, and antioxidant activities of the extract. The powdered stem bark was evaluated for its pharmacognostic profiles and then extracted successively using maceration method with methanol as solvent. The extract obtained was concentrated using rotary evaporator. AgNPs was characterized with the following machine such as UV/VIS, FTIR, SEM, and XRD. The extracts were screened for their phytochemical constituents using standard protocols. The thin layer chromatography (TLC) profiles of the resulting extracts were determined using suitable solvent systems and the retention factor (Rf) values of separated spots of compounds were calculated. The antioxidant (free radical scavenging) activity of the extracts were measured using DPPH (2, 2-diphenyl-1- picryl hydrazyl) and hydrogen peroxide with ascorbic acid used as standard. The results were expressed in terms of percentage inhibition. The preliminary phytochemical screening of the extracts revealed the presence of tannins, phenol, saponins, flavonoids and Alkaloids. The free radical scavenging activity of the extracts revealed higher activity with decrease in concentration in the nanoparticles compared to Methanol extract when DPPH was used while reverse was the case when hydrogen peroxide was used. Also, except in salmonella, antimicrobial Activity is higher in the nanoparticles compared with the methanol extract. The results indicated that the extracts contain secondary metabolites which may be responsible for the antioxidant and antimicrobial activity demonstrated by the extract of E. sengalensis and silver nanoparticles (AgNPs) with the plant mediated AgNPs found to also suppress the proliferation of multidrugresistant S. aureus.
650		0	_aFree radicals (Chemistry) _vDissertations, Academic
650		0	_a Anti-infective agents _vDissertations, Academic
650		0	_a Traditional medicine _vDissertations, Academic
700	1		_aHalilu, Emmanuel Mshella _esupervisor
942			_2ddc _cTS
999			_c288924 _d288924