000			02908nam a22003257a 4500
003			KOHA
005			20230424110419.0
008			221101d2022 cy ||||| m||| 00| 0 eng d
040			_aCY-NiCIU _beng _cCY-NiCIU _erda
041			_aeng
090			_aYL 2629 _bS45 2022
100	1		_aShittu, Ridwan Olanrewaju
245	1	0	_aCYTOTOXIC, ANTIOXIDANT AND ANTIMICROBIAL EFFECTS IF DANDELION ROOT EXTRACT (Taraxacum officinale) ON BREAST CANCER CELLS / _cRIDWAN OLANREWAJU SHITTU; SUPERVISOR: ASSIST. PROF. DR. PWADUBASHIYI COSTON PWAVODI
264			_c2022
300			_a43 sheets; _c31 cm. _eIncludes CD
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
502			_aThesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
504			_aIncludes bibliography (sheets 36-43)
520			_aABSTRACT Breast cancer (BC) is one of the leading causes of death globally, resulting to 2.26 million cases in the world. Among treatment used to down regulate this tumor cells include surgery, immunotherapy and chemotherapy. Although, there are habituation expressed by the tumor cells after using the later. Different plants have been used in the treatment of diseases ranging from bacterial infection to cancer. Among natural health products which has been confirmed to be potent and still undergoing clinical trials include dandelion plant which is also known as Taraxacum officinale. Characterization of Dandelion root extract (DRE) was evaluated by gas chromatography mass spectroscopy (GC-MS) device. Trypan blue exclusion assay was used to determine the cytotoxic effect on MDA- MB 231 cells, antioxidant activity using diphenylpicryl hydrazine (DPPH) scavenging assay and antimicrobial activity was assessed by disc diffusion assay. The GC-MS result revealed the presence of bioactive compounds like polyphenols, triterpenoids and flavonoids. In this study, it was shown that dandelion root extract (DRE) efficiently killed the tumor cells in a dose dependent manner. Antioxidant activities carried out using DPPH as stable radical shows IC50 value of 0.421 ± 0.001 mg/mL. DRE did not show any inhibitory effect against two gram negative and gram-positive bacterial pathogen. Our study shows that DRE has proven novel in killing breast cancer cells, mildly strong antioxidant activity to scavenge free radicals and no inhibitory effect against some bacteria.
650		0	_aAnti-infective agents _vDissertations, Academic
650		0	_aAntioxidants _vDissertations, Academic
650		0	_aBreast _vDissertations, Academic _xCancer
650		0	_aChemotherapy _vDissertations, Academic
650		0	_aDandelions _vDissertations, Academic
700	1		_aPwavodi, Pwadubashiyi Coston _esupervisor
942			_2ddc _cTS
999			_c288958 _d288958