000			03764nam a22003017a 4500
003			KOHA
005			20231030140940.0
008			231030d2023 cy ||||| m||| 00| 0 eng d
040			_aCY-NiCIU _beng _cCY-NiCIU _erda
041			_aeng
090			_aYL 3148 _bK25 2023
100	1		_aKakkaba, Amina Abubakar
245	1	0	_aPHARMACOGNOSTIC AND BIOLOGICAL STUDIES OF Artemisia absinthium L. LEAF / _cAMINA ABUBAKAR KAKKABA; SUPERVISOR: ASST. PROF. DR. EMMANUEL MSHELIA HALILU
264			_c2023
300			_axii, 71 sheets; _c31 cm. _e1 CD-ROM
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
502			_aThesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department
504			_aIncludes bibliography (sheets 58-71)
520			_aABSTRACT Artemisia absinthium L. (Wormwood) is a plant which belongs to the family Asteraceae and it has been used by traditional healers to treat many ailments and infections in African traditional medicine. It is popularly used in pharmaceutics to manufacture artemisinin-based combination therapy against malaria. There is a growing concern about the scourge of diseases caused by excessive free radicals in the body and lack of standardization of medicinal herbal products. Therefore, this research is aimed at evaluating the antibacterial and antioxidant activities of the extract. The powdered leaf powder was evaluated for its pharmacognostic profiles and then extracted successively using maceration method with methanol as solvent. AgNPs was characterized with the following techniques such as UV/VIS and FTIR. The extracts were screened for their phytochemical constituents using standard protocols. The preliminary phytochemical screening of the extracts revealed the presence of tannins, saponins, flavonoids and steroids and terpenoids. The thin layer chromatography (TLC) profiles of the resulting extracts were determined using suitable solvent systems. The petroleum ether extract showed 3 spots and the Rf values are 0.78, 0.44 and 0.14, whereas 8 spots were found in methanol extract with Rf values 0.90, 0.77, 0.58, 0.35, 0.29, 0.19, 0.12 and 0.08. The antioxidant (free radical scavenging) activity of the extracts was measured using DPPH (2, 2-diphenyl-1- picryl hydrazyl) with ascorbic acid used as standard. The IC50 of the ascorbic acid and the extract were 1.305mg/ml and 0.4128mg/ml respectively. The results were expressed in terms of percentage inhibition. The free radical scavenging activity of the extracts revealed lower activity and a subsequent increase then decline with decrease in concentration in the methanol extract when DPPH was used. The zone of inhibition for the extract, nanoparticles aare 0mm, 0.67mm and 9.33 for E. coli and 3.33mm, 1.67mm and 12.33mm for Bacillus subtillis respectively. The minimum inhibitory concentration (MIC) of extract, nanoparticles and standard antibiotic are 0.44µg/ml, 0.40µg/ml and 0.24µg/ml respectively. The results indicated that the extracts contain secondary metabolites which may be responsible for the antioxidant and antimicrobial activity demonstrated by the extract of A. absinthium L. and silver nanoparticles (AgNPs). Keywords: Antimicrobial characteristics, Antioxidant characteristics, Artemisia absinthium, Pharmacognostic characteristics.
650		0	_aAnti-infective agents _vDissertations, Academic
650		0	_aAntioxidants _vDissertations, Academic
650		0	_aAbsinth wormwood _vDissertations, Academic
650		0	_aPharmacognosy _vDissertations, Academic
700	1		_aHalilu, Emmanuel Mshelia _esupervisor
942			_2ddc _cTS
999			_c291591 _d291591