000			03456nam a22003017a 4500
003			KOHA
005			20231030141811.0
008			231030d2023 cy do||| m||| 00| 0 eng d
040			_aCY-NiCIU _beng _cCY-NiCIU _erda
041			_aeng
090			_aYL 3147 _bU46 2023
100	1		_aUkochovwera, Oghenetejeri
245	1	0	_aGREEN SYNTHESIS OF IRON NANOPRTICLES USING THE LEAF EXTRACT OF LAURUS NOBILIS L. AND ITS ASSESMENT OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES / _cUKOCHOVWERA OGHENETEJERI; SUPERVISOR: ASSIST. PROF.DR PWADUBASHIYI COSTON PWAVODI
264			_c2023
300			_aix, 58 sheets: _bcharts, photos; _c31 cm. _e1 CD-ROM
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
502			_aThesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
504			_aIncludes bibliography (sheets 51-58)
520			_aABSTRACT The synthesis of nanoparticles using biomaterials is known as green nanotechnology, and it aims to promote environmentally sound and long-lasting technologies. This study focuses on the straightforward and sustainable production of iron sulphate nanoparticles using an aqueous extract of Laurus nobilis L. leaves. X-ray diffraction analysis (XRD), Fourier transform infrared (FT-IR), and ultraviolet-visible (UV-visible) spectroscopy were used to characterize the nanoparticles. The outcomes demonstrated that the nanoparticles had a crystalline structure, were mostly spherical, and had only a small number of hexagonal particles. Zeta analysis determined that the average size of the nanoparticles was 60.0 nm. The extraction by maceration yield was 10.07% of the total yield. Additionally, six different bacterial species were used to examine the nanoparticles' antimicrobial effectiveness. The nanoparticles created using this green manufacturing approach showed promising antioxidant and antibacterial properties. Phytochemical analysis revealed the presence of tannins, flavonoids, saponins, alkaloids, and steroids, all of which are important in transforming iron sulfate into iron nanoparticles with antibacterial and antioxidant properties. With inhibition zones ranging from 15 to 25 mm, the extracts, and iron nanoparticles showed significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa, Listeria pneumonia. Using the MIC (minimum inhibitory concentration) assays, the effectiveness of Laurus nobilis extracts against the above-mentioned bacterial strains was assessed. MIC values for L. nobilis extracts were 0.201 0.01 mg/mL for L. pneumophila, 0.602 0.15 mg/mL for S. aureus, 0.654 0.04 mg/mL for E. coli, 0.51 0.02 mg/mL for E. faecalis, and 0.38 0.05 mg/mL for P. aeruginosa. Additionally, all of the examined microbes were resistant to the antibacterial effects of gentamycin and iron nanoparticle concentrations. Keywords: Antibacterial, Antioxidant, Green Synthesis, Laurus nobilis Nanotechnology, Phytochemical Screening
650		0	_aAntibacterial agents _vDissertations, Academic
650		0	_aAntioxidants _vDissertations, Academic
650		0	_aLaurus nobilis _vDissertations, Academic
650		0	_aNanotechnology _vDissertations, Academic
700	1		_aPwavodi, Pwadubabashiyi Coston _esupervisor
942			_2ddc _cTS
999			_c291592 _d291592