000			03132nam a22002657a 4500
003			KOHA
005			20241016091202.0
008			240923d2024 cy d a|| |||| 00| 0 eng d
040			_aCY-NiCIU _beng _cCY-NiCIU _erda
041			_aeng
090			_aYL 3441 _bC45 2024
100	1		_aChigbo, Peter Aloysius
245	1	0	_aPHYTOCHEMICAL SCREENING, GREEN SYNTHESIS AND CHARACTERIZATION OF IRON OXIDE NANOPARTICLES USING LEAF EXTRACT OF FICUS CARICA L. AND ITS ASSESSMENT OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY / _cPETER ALOYSIUS CHIGBO ; SUPERVISOR, DR. LUBNA ADIL KAMIL ALGAİLANİ
264			_c2024
300			_a125 sheets : _e+1 CD ROM _c30 cm _billusrtrations, tables ;
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
502			_aThesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering
520			_aRecent nanotechnology research has successfully synthesized iron oxide nanoparticles from various plant species using green synthetic methods, examining their bioactivity features. Ficus carica, a Moraceae plant found in Mediterranean regions and dry climates, is known for its medicinal properties and biologically active chemicals for treating various ailments including diabetes, diarrhea, respiratory diseases. The GC-MS analysis revealed that the n-Hexane extract contains 159 chemotypes, including fatty acids esters, sterols, unsaturated alcohols, and alkynes, all of which have significant phytopharmaceutical relevance. The study aims to synthesize iron oxide nanoparticles using Ficus carica leaf extracts, characterizing them using techniques like UV-visible spectroscopy, XRD, FT-IR, Zetasizer analysis, phytochemical examination, antibacterial and antioxidant activity. UV spectra analysis indicated the spectrum with the peaks of 315, 271, 224, and 198 nm with absorption while that of plant extract peaks are 317, 266, 224 and 215nm. The zeta size has a size of 77.37 nm with a charge of -17.01 mV. The study assessed antioxidant activity utilizing DPPH free radical and H2O2 scavenging tests on extract, iron oxide nanoparticles, iron oxide solution, and ascorbic acid. The IC50 of DPPH free scavenging activity of the extract, iron oxide nanoparticles, and ascorbic acid (standard) were 0.08744mg/mL, 0.9275mg/mL, and 0.0000509mg/mL respectively while the IC50 of hydrogen peroxide (H2O2) scavenging activity were 1.151mg/mL, 1.668mg/mL, and 0.6743mg/mL respectively. Ficus carica L extract demonstrated antibacterial activity against Staphylococcus aureus and Escherichia coli using the agar-well diffusion method, inhibiting growth with a zone of inhibition ranging between 7.016-8.660mm for nanoparticles, while standard drugs cefoxitin and gentamycin ranged between 22.69-28.86mm and 0.216-0.311mg/mL respectively, indicating potential as antioxidants and bacterial strain inhibitors.
650		0	_aBioengineering _vDissertations, Academic
700	1		_aAlgailani, Lubna Adıl Kamıl _esupervisor
942			_2ddc _cTS
999			_c292813 _d292813