000			03757nam a22002657a 4500
003			KOHA
005			20241009121143.0
008			240927d2024 cy e|||| |||| 00| 0 eng d
040			_aCY-NiCIU _beng _cCY-NiCIU _erda
041			_aeng
090			_aD 433 _bK67 2024
100	1		_aKorkmazer, Çağın
245	1	0	_aINVESTIGATION OF CHEMICAL AND PHARMACOLOGICAL ACTIVITIES OF EXTRACTS OF SCUTELLARIA SIBTHORPII (BENTH) HALACSY / _cÇAĞIN KORKMAZER ; SUPERVISOR, ASST. PROF. DR. EMMANUEL MSHELIA HALILU
264			_c2024
300			_a114 sheets ; _c30 cm _e+1 CD ROM
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
502			_aThesis (PhD) - Cyprus International University. Institute of Graduate Studies and Research Pharmaceutıcal Sciences
520			_aScutellaria sibthorpii is an endemic plant of Cyprus and it is used in the treatment of microbial infections, pains and inflammations. The powder leaf was extracted by maceration in 80% methanol for 24 hours and then fractionated using n-hexane, ethyl acetate and n-butanol. The crude extract and fractions were screened for the presence of saponins, tannins, flavonoids and triterpenoids/steroids. Total phenolic and flavonoid contents were determined using Folin–Ciocalteu’s reagent and aluminium chloride assays. The chemical profiling of ethyl acetate fraction, n-butanol and the crude extract were conducted by LC- MS/ LC-QTOF-MS based metabolomic profile analyses. The essential oil was analysed by GC-MS and revealed the presence of monoterpenoids, sesquiterpenoids and other compounds. The antioxidant activity was determined using DPPH, hydrogen peroxide and β-carotene assays. Agar disk diffusion and micro broth dilution methods were used for the antibacterial activity of the crude extract on Pseudomonas aeruginosa and Legionella pneumophila. Gentamicin (10 μg) was used as a positive control. The acute toxicity was determined using Lorke’s method. The analgesic activity assayed using acetic acid-induced writhing, hotplate and formalin-induced nociception. Anti-inflammatory activity was investigated by means of formalin-induced acute inflammation. The antipyretic activity was studied using Brewer’s yeast. The crude extract (CrESs)/fractions demonstrated antioxidant activity in all the models. The nhexane fraction (HFSs) had the highest scavenging activity with IC50 of 2.944 m/mL in the DPPH assay. In the H2O2 scavenging assay, the ethyl acetate fraction (EtFSs) had the highest scavenging activity (IC50 of 0.4352 mg/mL) and in the β-carotene bleaching assay with IC50 of 0.00014 mg/mL. Generally, the EtFSs had higher antioxidant activity when compared with other test samples and with ascorbic acid (2.944 mg/mL-Hexane, 9.29 mg/mL-hydrogen peroxide and 2.85 mg/mL-β-carotene). The MIC of CrESs on Pseudomonas aeruginosa and Legionella pneumophila were 0.15625±0.00 mg/mL and 0.3125±0.00 mg/mL respectively. The LD50 of the CrESs /fractions were greater than 5000 mg/Kg. The CrESs/fractions demonstrated analgesic activity but it was higher in EtFSs when compared to piroxicam. The EtFSs showed higher anti-inflammatory activity than piroxicam at the late hours. The antipyretic study revealed at 200 mg/Kg, EtFSs fraction significantly reduced the rectal temperature of rats similar to paracetamol. The findings of this research have validated the uses of Scutellaria sibthorpii in traditional medicine as antibacterial, antioxidant, analgesic, anti-inflammatory and antipyretic.
650		0	_aPharmaceutıcal Sciences _vDissertations, Academic
700	1		_aHalilu, Emmanuel Mshelia _esupervisor
942			_2ddc _cTS
999			_c292893 _d292893