000			03511nam a22002657a 4500
003			KOHA
005			20250110105514.0
008			240925d2024 cy oed|| |||| 00| 0 eng d
040			_aCY-NiCIU _beng _cCY-NiCIU _erda
041			_aeng
090			_aYL 3551 _bO58 2024
100	1		_aOnuh, Joy Enemona
245	1	0	_aCOMPARATIVE PHYTOCHEMICAL AND ANTIOXIDANT ANALYSIS OF AQUEOUS/METHANOL LEAF EXTRACTS OF THREE SPECIES OF LAVENDER / _cJOY ENEMONA ONUH ; SUPERVISOR, ASST. PROF. DR. EMMANUEL MSHELIA HALILU
264			_c2024
300			_a109 sheets; _c30 cm _e+1 CD ROM
336			_2rdacontent _atext _btxt
337			_2rdamedia _aunmediated _bn
338			_2rdacarrier _avolume _bnc
502			_aThesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy
520			_aLavender belongs to the lamiaceae family and consists of 47 known species. These species are characterized by their distinct aroma and have been utilized for their therapeutic properties. The study was aimed to comparatively investigate the botanical, physicochemical, phytochemical and antioxidant properties of Lavandula dentata, Lavandula stoechas and Lavandula angustifolia. The various plant extracts were obtained by maceration in 80% methanol and water, and further screened for the presence of some secondary metabolites using standard procedures. The total phenolic and total flavonoid contents were estimated using Folin-Ciocalteu reagent and AlCl3 assays respectively. The essential oil was extracted using the Clevenger apparatus and the constituent terpenoids were analyzed using GC-MS. The antioxidant activity of the extracts and the essential oils was assayed using DPPH free radical. Macroscopically, the three plant species were characteristically different on the basis of leaves amongst others. Microscopically, trichomes and stoma of various forms were observed from the upper and lower epidermis. The moisture content, total and acid insoluble ash values, hexane, alcohol and water soluble extractive value of the three species were found to be within the acceptable limit. The % yield of the crude methanol extracts for Lavandula dentata, Lavandula stoechas and Lavandula angustifolia are 26%, 31.6% and 27.8%, the % yield of the water extracts were: 17.6%, 22.3%, 15.1% and the % yield of the essential oils were: 5.17%, 2.16%, and 5.06% respectively as in the above order. The phytochemical analysis revealed saponins, phenolic groups, tannins, flavonoids, alkaloids, cardiac glycosides, steroids and triterpenoids. The total phenolic contents ranged between 0.1010-0.2483 mg GAE/g for all the test samples. The total flavonoids ranged between 0.0810-0.1929 mg QE/g. The GC-MS analysis of the essential oils revealed β-pinene, 1,8-cineole, fenchone, camphor, α-terpineol and sabinene. The antioxidant activity was expressed in terms of IC50 and on this, the Lavandula dentata water extract had the highest activity with IC50 of 4.065 mg/mL and the activity was found to be better than ascorbic acid with IC50 66.35 mg/mL. The overall results showed that Lavender species contains phytochemicals with antioxidants activities which justifies their usage in traditional medicine for the prevention and treatment of diseases whose causes are associated with free radicals.
650		0	_aPharmacognosy _vDissertations, Academic
700	1		_aHalilu, Emmanuel Mshelia _esupervisor
942			_2ddc _cTS
999			_c293096 _d293096