GREEN SYNTHESIS OF IRON NANOPRTICLES USING THE LEAF EXTRACT OF LAURUS NOBILIS L. AND ITS ASSESMENT OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES /
Ukochovwera, Oghenetejeri
GREEN SYNTHESIS OF IRON NANOPRTICLES USING THE LEAF EXTRACT OF LAURUS NOBILIS L. AND ITS ASSESMENT OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES / UKOCHOVWERA OGHENETEJERI; SUPERVISOR: ASSIST. PROF.DR PWADUBASHIYI COSTON PWAVODI - ix, 58 sheets: charts, photos; 31 cm. 1 CD-ROM
Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
Includes bibliography (sheets 51-58)
ABSTRACT
The synthesis of nanoparticles using biomaterials is known as green nanotechnology, and it aims
to promote environmentally sound and long-lasting technologies. This study focuses on the
straightforward and sustainable production of iron sulphate nanoparticles using an aqueous
extract of Laurus nobilis L. leaves. X-ray diffraction analysis (XRD), Fourier transform infrared
(FT-IR), and ultraviolet-visible (UV-visible) spectroscopy were used to characterize the
nanoparticles. The outcomes demonstrated that the nanoparticles had a crystalline structure,
were mostly spherical, and had only a small number of hexagonal particles. Zeta analysis
determined that the average size of the nanoparticles was 60.0 nm. The extraction by maceration
yield was 10.07% of the total yield. Additionally, six different bacterial species were used to
examine the nanoparticles' antimicrobial effectiveness. The nanoparticles created using this
green manufacturing approach showed promising antioxidant and antibacterial properties.
Phytochemical analysis revealed the presence of tannins, flavonoids, saponins, alkaloids, and
steroids, all of which are important in transforming iron sulfate into iron nanoparticles with
antibacterial and antioxidant properties. With inhibition zones ranging from 15 to 25 mm, the
extracts, and iron nanoparticles showed significant antibacterial activity against Escherichia coli,
Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa,
Listeria pneumonia. Using the MIC (minimum inhibitory concentration) assays, the
effectiveness of Laurus nobilis extracts against the above-mentioned bacterial strains was
assessed. MIC values for L. nobilis extracts were 0.201 0.01 mg/mL for L. pneumophila, 0.602
0.15 mg/mL for S. aureus, 0.654 0.04 mg/mL for E. coli, 0.51 0.02 mg/mL for E. faecalis, and
0.38 0.05 mg/mL for P. aeruginosa. Additionally, all of the examined microbes were resistant
to the antibacterial effects of gentamycin and iron nanoparticle concentrations.
Keywords: Antibacterial, Antioxidant, Green Synthesis, Laurus nobilis
Nanotechnology, Phytochemical Screening
Antibacterial agents--Dissertations, Academic
Antioxidants--Dissertations, Academic
Laurus nobilis--Dissertations, Academic
Nanotechnology--Dissertations, Academic
GREEN SYNTHESIS OF IRON NANOPRTICLES USING THE LEAF EXTRACT OF LAURUS NOBILIS L. AND ITS ASSESMENT OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES / UKOCHOVWERA OGHENETEJERI; SUPERVISOR: ASSIST. PROF.DR PWADUBASHIYI COSTON PWAVODI - ix, 58 sheets: charts, photos; 31 cm. 1 CD-ROM
Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department
Includes bibliography (sheets 51-58)
ABSTRACT
The synthesis of nanoparticles using biomaterials is known as green nanotechnology, and it aims
to promote environmentally sound and long-lasting technologies. This study focuses on the
straightforward and sustainable production of iron sulphate nanoparticles using an aqueous
extract of Laurus nobilis L. leaves. X-ray diffraction analysis (XRD), Fourier transform infrared
(FT-IR), and ultraviolet-visible (UV-visible) spectroscopy were used to characterize the
nanoparticles. The outcomes demonstrated that the nanoparticles had a crystalline structure,
were mostly spherical, and had only a small number of hexagonal particles. Zeta analysis
determined that the average size of the nanoparticles was 60.0 nm. The extraction by maceration
yield was 10.07% of the total yield. Additionally, six different bacterial species were used to
examine the nanoparticles' antimicrobial effectiveness. The nanoparticles created using this
green manufacturing approach showed promising antioxidant and antibacterial properties.
Phytochemical analysis revealed the presence of tannins, flavonoids, saponins, alkaloids, and
steroids, all of which are important in transforming iron sulfate into iron nanoparticles with
antibacterial and antioxidant properties. With inhibition zones ranging from 15 to 25 mm, the
extracts, and iron nanoparticles showed significant antibacterial activity against Escherichia coli,
Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa,
Listeria pneumonia. Using the MIC (minimum inhibitory concentration) assays, the
effectiveness of Laurus nobilis extracts against the above-mentioned bacterial strains was
assessed. MIC values for L. nobilis extracts were 0.201 0.01 mg/mL for L. pneumophila, 0.602
0.15 mg/mL for S. aureus, 0.654 0.04 mg/mL for E. coli, 0.51 0.02 mg/mL for E. faecalis, and
0.38 0.05 mg/mL for P. aeruginosa. Additionally, all of the examined microbes were resistant
to the antibacterial effects of gentamycin and iron nanoparticle concentrations.
Keywords: Antibacterial, Antioxidant, Green Synthesis, Laurus nobilis
Nanotechnology, Phytochemical Screening
Antibacterial agents--Dissertations, Academic
Antioxidants--Dissertations, Academic
Laurus nobilis--Dissertations, Academic
Nanotechnology--Dissertations, Academic