PHYTOCHEMICAL, AND SYNTHESIS OF SILVER NANOPARTICLES FROM CITRUS RETICULATA PEELS AND ITS ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY / AHLAM LAABOUDI; SUPERVISOR: ASSY. PROF. DR. EMMANUEL MSHELLA HALILU

Yazar: Katkıda bulunan(lar):Dil: İngilizce 2022Tanım: 50 sheets; 31 cm. Includes CDİçerik türü:
  • text
Ortam türü:
  • unmediated
Taşıyıcı türü:
  • volume
Konu(lar): Tez notu: Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department Özet: ABSTRACT Tangerine (Citrus reticulata L.) is a type of citrus plant. Citrus is a genus of flowering plants in the family Rutaceae and a common name for edible fruits of this genus. Citrus contains phenolic bioactive compounds which confirred a powerful antioxidant, antiinflammatory and anticancer properties.Therefore this research is aimed to evaluate the phytochemical and antioxidant effect of tangerine peels extracts/syilver nanoparticles. The Tangerine peels powder was evalueted for its pharmacognostic properties and then extracted with methanol using maceration and the extract was subjected to the phytochemical studies. The thin layer chromatography (TLC) were conducted using different solvent systems and the Rf values of the separeted component were calculated . The synthesis of Nanoparticles was made using Silver nitrate (AgNO3), and the nanoparticles was charactarized using FT-IR and SEM analysis. The antioxidant activity of the extract were determined using DPPH and Hydrogen peroxide Scavenging Assay (HPSA) using ascorbic acid as standard. The antibacterial activity of the extract and silver nanoparticles was performed using agar diffusion method against Escherichia coli and Staphylococcus aureus. The premilinary phytochemical screening of the extract indicate the presence of tanins,saponins,alkaloids and flavonoids . The free radical scavening activity of the plant extract showed IC50 of 6.17 mg/ml using DPPH, and IC50 of 8.4 mg/ml using HPSA. The ascorbic acid (standard) showed IC50 = 1.929 mg/ml. The Antibacterial test releaved that the tangerine peels plant extract had an antibacteerial activity against the Gram postive Staphylococcus aureus and no activity against Escherichia coli .The results indicated that the extract contain phenolic coumpounds that can scavenge free radicals and inbibit the growth of Gram postive bacteria. Keywords: Amtibacterial, Antioxidant ,Citrus reticulata, DPPH
Materyal türü: Thesis
Mevcut
Materyal türü Geçerli Kütüphane Koleksiyon Yer Numarası Durum Notlar İade tarihi Barkod Materyal Ayırtmaları
Thesis Thesis CIU LIBRARY Tez Koleksiyonu Tez Koleksiyonu YL 2564 L23 2022 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Pharmacognosy Department T2885
Suppl. CD Suppl. CD CIU LIBRARY Görsel İşitsel YL 2564 L23 2022 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Pharmacognosy Department CDT2885
Toplam ayırtılanlar: 0

Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department

Includes bibliography (sheets 47-50)

ABSTRACT
Tangerine (Citrus reticulata L.) is a type of citrus plant. Citrus is a genus of flowering
plants in the family Rutaceae and a common name for edible fruits of this genus. Citrus
contains phenolic bioactive compounds which confirred a powerful antioxidant, antiinflammatory
and anticancer properties.Therefore this research is aimed to evaluate
the phytochemical and antioxidant effect of tangerine peels extracts/syilver
nanoparticles. The Tangerine peels powder was evalueted for its pharmacognostic
properties and then extracted with methanol using maceration and the extract was
subjected to the phytochemical studies. The thin layer chromatography (TLC) were
conducted using different solvent systems and the Rf values of the separeted
component were calculated . The synthesis of Nanoparticles was made using Silver
nitrate (AgNO3), and the nanoparticles was charactarized using FT-IR and SEM
analysis. The antioxidant activity of the extract were determined using DPPH and
Hydrogen peroxide Scavenging Assay (HPSA) using ascorbic acid as standard. The
antibacterial activity of the extract and silver nanoparticles was performed using agar
diffusion method against Escherichia coli and Staphylococcus aureus. The
premilinary phytochemical screening of the extract indicate the presence of
tanins,saponins,alkaloids and flavonoids . The free radical scavening activity of the
plant extract showed IC50 of 6.17 mg/ml using DPPH, and IC50 of 8.4 mg/ml using
HPSA. The ascorbic acid (standard) showed IC50 = 1.929 mg/ml. The Antibacterial
test releaved that the tangerine peels plant extract had an antibacteerial activity against
the Gram postive Staphylococcus aureus and no activity against Escherichia coli .The
results indicated that the extract contain phenolic coumpounds that can scavenge free
radicals and inbibit the growth of Gram postive bacteria.
Keywords: Amtibacterial, Antioxidant ,Citrus reticulata, DPPH

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