EVALUATION OF CHEMICAL, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF Erythrina senegalensis D.C STEM BARK EXTRACT / EMMANUEL OMOLAN; SUPERVISOR: ASST. PROF. DR. EMMANUEL MSHELIA HALILU

Yazar: Katkıda bulunan(lar):Dil: İngilizce 2022Tanım: 91 sheets; 31 cm. Includes CDİçerik türü:
  • text
Ortam türü:
  • unmediated
Taşıyıcı türü:
  • volume
Konu(lar): Tez notu: Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department Özet: ABSTRACT Erythrina senegalensis, the Senegal coral tree is a plant which belongs to the family of Fabaceae and it has been used by traditional healers to treat many ailments and infections in African traditional medicine. There is a growing concern about the scourge of diseases caused by excessive free radicals in the body and lack of standardization of medicinal herbal products. Therefore, this research is aimed at evaluating the chemical, antibacterial, and antioxidant activities of the extract. The powdered stem bark was evaluated for its pharmacognostic profiles and then extracted successively using maceration method with methanol as solvent. The extract obtained was concentrated using rotary evaporator. AgNPs was characterized with the following machine such as UV/VIS, FTIR, SEM, and XRD. The extracts were screened for their phytochemical constituents using standard protocols. The thin layer chromatography (TLC) profiles of the resulting extracts were determined using suitable solvent systems and the retention factor (Rf) values of separated spots of compounds were calculated. The antioxidant (free radical scavenging) activity of the extracts were measured using DPPH (2, 2-diphenyl-1- picryl hydrazyl) and hydrogen peroxide with ascorbic acid used as standard. The results were expressed in terms of percentage inhibition. The preliminary phytochemical screening of the extracts revealed the presence of tannins, phenol, saponins, flavonoids and Alkaloids. The free radical scavenging activity of the extracts revealed higher activity with decrease in concentration in the nanoparticles compared to Methanol extract when DPPH was used while reverse was the case when hydrogen peroxide was used. Also, except in salmonella, antimicrobial Activity is higher in the nanoparticles compared with the methanol extract. The results indicated that the extracts contain secondary metabolites which may be responsible for the antioxidant and antimicrobial activity demonstrated by the extract of E. sengalensis and silver nanoparticles (AgNPs) with the plant mediated AgNPs found to also suppress the proliferation of multidrugresistant S. aureus.
Materyal türü: Thesis
Mevcut
Materyal türü Geçerli Kütüphane Koleksiyon Yer Numarası Durum Notlar İade tarihi Barkod Materyal Ayırtmaları
Thesis Thesis CIU LIBRARY Tez Koleksiyonu Tez Koleksiyonu YL 2511 O46 2022 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Pharmacognosy Department T2832
Suppl. CD Suppl. CD CIU LIBRARY Görsel İşitsel YL 2511 O46 2022 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Pharmacognosy Department CDT2832
Toplam ayırtılanlar: 0

Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Pharmacognosy Department

Includes bibliography (sheets 73-91)

ABSTRACT
Erythrina senegalensis, the Senegal coral tree is a plant which belongs to the family
of Fabaceae and it has been used by traditional healers to treat many ailments and
infections in African traditional medicine. There is a growing concern about the
scourge of diseases caused by excessive free radicals in the body and lack of
standardization of medicinal herbal products. Therefore, this research is aimed at
evaluating the chemical, antibacterial, and antioxidant activities of the extract. The
powdered stem bark was evaluated for its pharmacognostic profiles and then
extracted successively using maceration method with methanol as solvent. The
extract obtained was concentrated using rotary evaporator. AgNPs was characterized
with the following machine such as UV/VIS, FTIR, SEM, and XRD. The extracts
were screened for their phytochemical constituents using standard protocols. The thin
layer chromatography (TLC) profiles of the resulting extracts were determined using
suitable solvent systems and the retention factor (Rf) values of separated spots of
compounds were calculated. The antioxidant (free radical scavenging) activity of the
extracts were measured using DPPH (2, 2-diphenyl-1- picryl hydrazyl) and hydrogen
peroxide with ascorbic acid used as standard. The results were expressed in terms of
percentage inhibition. The preliminary phytochemical screening of the extracts
revealed the presence of tannins, phenol, saponins, flavonoids and Alkaloids. The
free radical scavenging activity of the extracts revealed higher activity with decrease
in concentration in the nanoparticles compared to Methanol extract when DPPH was
used while reverse was the case when hydrogen peroxide was used. Also, except in
salmonella, antimicrobial Activity is higher in the nanoparticles compared with the
methanol extract. The results indicated that the extracts contain secondary
metabolites which may be responsible for the antioxidant and antimicrobial activity
demonstrated by the extract of E. sengalensis and silver nanoparticles (AgNPs) with
the plant mediated AgNPs found to also suppress the proliferation of multidrugresistant
S. aureus.

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