Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Chemistry

A class of chemical compounds known as indole and its derivatives are one of the
important pharmacophores in the field of medical chemistry. These compounds have
distinct structural characteristics that enable them to engage in a variety of important
biological activities. By varying the location and kind of substituent, indole
derivatives' biological activity can be adjusted. As a result, they have been focus of
in-depth research and use in the field of development and discovery of new drugs.
The flexibility in substitution on indoles provides a powerful tool to explore and
optimize the potential biological effects of these compounds. It opens up possibilities
for designing molecules with enhanced therapeutic properties and targeted
interactions with biological systems. The ability to conveniently modulate the
activities of indole derivatives through structural modifications makes them valuable
candidates for organic synthesis and drug design.
In this work, an indole-piperazine derivative was prepared under reflux condition
through Mannich reaction to yield a 3-methylindole substituted by phenylpiperazine
ring on the N-atom of the indole ring. Proton Nuclear Magnetic Resonance
Spectroscopy (1H-NMR) and Fourier Transform Infra-Red (FT-IR) were used to
characterize the target molecule. The FT-IR spectra of the synthesized molecule,
showed the absence of N-H stretch which indicated that the reaction has actually
taken place at position 1. Also the Mass Spectrometry result (ESI-MS) was used to
confirm the molecular weight of the synthesized molecule and its fragmentation. To
examine the purity of the synthesized molecule, Thin Layer Chromatography (TLC)
and melting point were conducted. Disk diffusion technique was also carried out for
antimicrobial susceptibility studies. The disk diffusion technique showed low to
moderate activity to gram positive (Bacillus) and gram negative ( E. coli)
microorganism.