GREEN SYNTHESIS OF IRON NANOPRTICLES USING THE LEAF EXTRACT OF LAURUS NOBILIS L. AND ITS ASSESMENT OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES / UKOCHOVWERA OGHENETEJERI; SUPERVISOR: ASSIST. PROF.DR PWADUBASHIYI COSTON PWAVODI

Yazar: Katkıda bulunan(lar):Dil: İngilizce 2023Tanım: ix, 58 sheets: charts, photos; 31 cm. 1 CD-ROMİçerik türü:
  • text
Ortam türü:
  • unmediated
Taşıyıcı türü:
  • volume
Konu(lar): Tez notu: Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department Özet: ABSTRACT The synthesis of nanoparticles using biomaterials is known as green nanotechnology, and it aims to promote environmentally sound and long-lasting technologies. This study focuses on the straightforward and sustainable production of iron sulphate nanoparticles using an aqueous extract of Laurus nobilis L. leaves. X-ray diffraction analysis (XRD), Fourier transform infrared (FT-IR), and ultraviolet-visible (UV-visible) spectroscopy were used to characterize the nanoparticles. The outcomes demonstrated that the nanoparticles had a crystalline structure, were mostly spherical, and had only a small number of hexagonal particles. Zeta analysis determined that the average size of the nanoparticles was 60.0 nm. The extraction by maceration yield was 10.07% of the total yield. Additionally, six different bacterial species were used to examine the nanoparticles' antimicrobial effectiveness. The nanoparticles created using this green manufacturing approach showed promising antioxidant and antibacterial properties. Phytochemical analysis revealed the presence of tannins, flavonoids, saponins, alkaloids, and steroids, all of which are important in transforming iron sulfate into iron nanoparticles with antibacterial and antioxidant properties. With inhibition zones ranging from 15 to 25 mm, the extracts, and iron nanoparticles showed significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa, Listeria pneumonia. Using the MIC (minimum inhibitory concentration) assays, the effectiveness of Laurus nobilis extracts against the above-mentioned bacterial strains was assessed. MIC values for L. nobilis extracts were 0.201 0.01 mg/mL for L. pneumophila, 0.602 0.15 mg/mL for S. aureus, 0.654 0.04 mg/mL for E. coli, 0.51 0.02 mg/mL for E. faecalis, and 0.38 0.05 mg/mL for P. aeruginosa. Additionally, all of the examined microbes were resistant to the antibacterial effects of gentamycin and iron nanoparticle concentrations. Keywords: Antibacterial, Antioxidant, Green Synthesis, Laurus nobilis Nanotechnology, Phytochemical Screening
Materyal türü: Thesis
Mevcut
Materyal türü Geçerli Kütüphane Koleksiyon Yer Numarası Durum Notlar İade tarihi Barkod Materyal Ayırtmaları
Thesis Thesis CIU LIBRARY Tez Koleksiyonu Tez Koleksiyonu YL 3147 U46 2023 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Bioengineering Department T3528
Suppl. CD Suppl. CD CIU LIBRARY Görsel İşitsel YL 3147 U46 2023 (Rafa gözat(Aşağıda açılır)) Kullanılabilir Bioengineering Department CDT3528
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Thesis (MSc) - Cyprus International University. Institute of Graduate Studies and Research Bioengineering Department

Includes bibliography (sheets 51-58)

ABSTRACT
The synthesis of nanoparticles using biomaterials is known as green nanotechnology, and it aims
to promote environmentally sound and long-lasting technologies. This study focuses on the
straightforward and sustainable production of iron sulphate nanoparticles using an aqueous
extract of Laurus nobilis L. leaves. X-ray diffraction analysis (XRD), Fourier transform infrared
(FT-IR), and ultraviolet-visible (UV-visible) spectroscopy were used to characterize the
nanoparticles. The outcomes demonstrated that the nanoparticles had a crystalline structure,
were mostly spherical, and had only a small number of hexagonal particles. Zeta analysis
determined that the average size of the nanoparticles was 60.0 nm. The extraction by maceration
yield was 10.07% of the total yield. Additionally, six different bacterial species were used to
examine the nanoparticles' antimicrobial effectiveness. The nanoparticles created using this
green manufacturing approach showed promising antioxidant and antibacterial properties.
Phytochemical analysis revealed the presence of tannins, flavonoids, saponins, alkaloids, and
steroids, all of which are important in transforming iron sulfate into iron nanoparticles with
antibacterial and antioxidant properties. With inhibition zones ranging from 15 to 25 mm, the
extracts, and iron nanoparticles showed significant antibacterial activity against Escherichia coli,
Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa,
Listeria pneumonia. Using the MIC (minimum inhibitory concentration) assays, the
effectiveness of Laurus nobilis extracts against the above-mentioned bacterial strains was
assessed. MIC values for L. nobilis extracts were 0.201 0.01 mg/mL for L. pneumophila, 0.602
0.15 mg/mL for S. aureus, 0.654 0.04 mg/mL for E. coli, 0.51 0.02 mg/mL for E. faecalis, and
0.38 0.05 mg/mL for P. aeruginosa. Additionally, all of the examined microbes were resistant
to the antibacterial effects of gentamycin and iron nanoparticle concentrations.
Keywords: Antibacterial, Antioxidant, Green Synthesis, Laurus nobilis
Nanotechnology, Phytochemical Screening

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